Wednesday, December 8, 2010

Micro Centrifuge Machine~the small skull in the lab

Trouble? 


Hope you guys were not frustrated by the exam this morning~~~but whats over is over
lets move on with our cutest machine in the lab~!!!
the~~~~~~~~

MICRO-CENTRIFUGE MACHINE~!
or you can call him the small skull

centrifuge is a piece of equipment, generally driven by an electric motor (some older models were spun by hand), that puts an object in rotation around a fixed axis, applying a force perpendicular to the axis. This machine is capable of spinning to a speed of upto 13.4rpm~! considering the size of it, i would say it is quite a amazing machine.

 ok~now for the ever famous Minispin~tadaaa
The one we using here is made by eppendorf. If i am not wrong, this is made in Germany. and they specially ship 10 of them over to Malaysia for us~!wahahah
ok~it have basically 7 button on the front panel. They were used to control the speed, the time and some basic setting such as short spin, start and a open~

below is what happen after i press the open button. The top of the skull is pop open, revealing the brain inside~!
the metal cap he is wearing is used to cover the tube that were spining in high speed inside. despise the size of it, i would say the cap is heavier than a normal fully packed pencil case. I think it would help to increase the stability of the machine. Eventhough the machine can still be functional without using the cap,(my tubes were way to big that the caps doesnt fit) it was totally not recommended. the noise were much louder even for a 3000rpm spin.

this is what ahppen when you splice open the brain. 12 little pockets of holes can be seen. The brain is currently empty. Yes, empty, but please dont call the machine stupid, you might broke his heart.

wait..he dont have a heart~!wahahah
ok~you might break his head if you think he is stupid.

an overview of a EMPTY brain chamber of secrets. With a number ranging from 1 to 12, who knows, one of it might contains the sorceress's stone. 

ok~back to the topic, the holes here are used to place a tube named microcentrifuge tubes, they will be discuss as below. 

These tubes were design to hold a capacity of 1.5ml, and has a air tight cap to prevent leaking even at violent shaking. and immersion water bath.

another view of micro-centrifuge tube under moonlight~~

now to show you how do you use them together with the microcentifuge machine~~

ATTENTION ATTENTION ATTENTION
NEVER PUT YOU MICRO CENTRIFUGE AT THIS POSITION 
YOU MIGHT MAKE THE MACHINE EXPLODE AT HIGH VELOCITY
SPRAYING SCRAP METAL ALL OVER THE PLACE~!!!!

NOW~for the correct positioning of the micro centrifuge tubes~
since this machine is base on the principle of centripetal force, all weight must be balance in order for it to operate correctly~
thanks for your attention


now is the real thing in action~
the little skull is now spinning at 5000rpm, and it continue for 4 minutes before it was automatically shut down~

now for a another spin at 13000rpm, it might seems very scary to make it running at this velocity~
but it is totally conman in research when you need to isolate your DNA sample~

yaya..like those people in CSI



OK~thats for today~was having a big exam this early morning and am now really hoping for a nap~


good night everyone~spicy dreams

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