HARPACTIRINAE BIOTECHNOLOGY: Gram stain~~

Hello everyone~

Since gram staining is always the first thing that is mention in the identification of a bacterial organism. today's post will be about how you are going to made a gram stain for your precious bacteria~~~

Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positiveand Gram-negative) based on the chemical and physical properties of their cell walls. Gram positive bacteria such as staphylococcus are usually much more resistant than gram negative bacteria such as E.coli。

This explain why staphylococcus epidermis, a gram negative bacteria were able to live on the dry and harsh condition on your skin (yes, your skin!!!), while E.coli normally colonized places which are much more moisture and dark.

Today organism will be~~~~~kaboom----------------our always famous E.coil.

On a coliform agar. This color of this agar indicates that E.coli is a type of coli form bacteria. btw, what exactly is a coliform!!?? tell you next time~wahahahha

so...below is a basic procedure of gram stainig~it is stated as below to only use 30 seconds for each steps, however, i used almost 1 minutes for all the staining actually.

Firstly,you will need to fixed your bacteria on your slides, which involve scooping up a bunch of bacteria and emulsified them in distilled water. After that, flame the slide on a flame until it appear to be dry enough to proceed~~

Now， as the stated in its name, crystal violet is a purple colored dye with a shinny look~however you were not able to make crystal out of it.

Next, iodine solution. the one that forms a dark blue color on any starch containing material such as tissue paper

Acetone, a common solution found in your NAIL POLISH REMOVER. Highly flammable and dangerous.

Safranin, a pinkish color dye which are very popular among the girls in the class. Sadly, You are NOT ALLOW TO USE THEM AS A NAIL POLISH,

Now, lets start~~~~

After you had flame dry your slides~ placed them on a rack so that they are easily accessible.

Apologize since i forget to took photos for crystal violet addition. The photo above shows the addition of iodine into the slides,the volume should just enough to cover your culture, the person is a bit wasteful~~~(Not me not me！)