SSBC annual dinner~

Hello~!!!!!!!~exam is near, and the stress level "should be" increasing for everyone~

So why not we take a  break from all the frustrating exam and have a Kit Kat in SSBC ANNUAL DINNER
this year, rather than having it on a random location, it had been decide to have it in school as it would be much for comfortable as the school is regard as our second home already

Date: 26 November 2010
Location:Swinburne sarawak MPH
Time: Theoretically is from 6-10

Some junk food for the kids living in every people hearts.The large chips was brought in the super market in spring, 4 dollar each~~~some others are picked up some where around the kuching~

Drinks for tonight, A perfect combination of orange juice and rose syrup, orange provide most of the taste while the rose provide the bloody color. Yes, to suite the theme for biotech

Potato salad from our Germen lecturer, a nice cold dish~~~but i have no idea how to make this

some Paku (a kind of local vegetable) with belacan (local spices), 

Another vege~~~ordered from the coffee shop just beside our school 

A super-sized beehon enough for 20 people

yes~no Photoshop on this picture. the girl on the background was already hungry after she saw this beehon

There is also buns from a bread shop located in Malaysia~~

of course there is much more than this~such as cakes, roti canai, soft drinks, fried rice and roasted chicken, but i was quite busy to take photos for all of them~~so sad~~~

Now, this is the oldest people around~they were named "1st batch"

since they were the 1st batch who enter biotech in this school

now for some pretty girls~~~

and for the mixed batch~not lecturers are found in this photo

especially the one having a peace sign  

And another mixed batch~~~~a passbyer can be observed at the right side~and yes, she is a biotech 

some games were also provide to ease the fully filled stomach~~today, only some simple game like bingo and puzzle are played~~just that this picture is drawn (by me,~~hehe, i know i suck in drawing skill) on the back of the bingo paper, and is being recombined by various genius after the bingo game.

A group photo later~this event was ended in a sophisticated way~

just to clarify~i only prepare the dance for one and a half minutes~and i played the wrong music.....
so the last part is kinda repetitive and messy~hope you all dont mind~
wahaha


okok~~time for me to rest my tiring arm and legs~~see you nect time

Gram stain~~

Hello everyone~

Since gram staining is always the first thing that is mention in the identification of a bacterial organism. today's post will be about how you are going to made a gram stain for your precious bacteria~~~

Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positiveand Gram-negative) based on the chemical and physical properties of their cell walls. Gram positive bacteria such as staphylococcus are usually much more resistant than gram negative bacteria such as E.coli。 

This explain why staphylococcus epidermis, a gram negative bacteria were able to live on the dry and harsh condition on your skin (yes, your skin!!!), while E.coli normally colonized places which are much more moisture and dark. 

Today organism will be~~~~~kaboom----------------our always famous E.coil.

On a coliform agar. This color of this agar indicates that E.coli is a type of coli form bacteria. btw, what exactly is a coliform!!?? tell you next time~wahahahha

so...below is  a basic procedure of gram stainig~it is stated as below to only use 30 seconds for each steps, however, i used almost 1 minutes for all the staining actually.

Firstly,you will need to fixed your bacteria on your slides, which involve scooping up a bunch of bacteria and emulsified them in distilled water. After that, flame the slide on a flame until it appear to be dry enough to proceed~~

Now， as the stated in its name, crystal violet is a purple colored dye with a shinny look~however you were not able to make crystal out of it.

 Next, iodine solution. the one that forms a dark blue color on any starch containing material such as tissue paper

Acetone, a common solution found in your NAIL POLISH REMOVER. Highly flammable and dangerous.  

Safranin, a pinkish color dye which are very popular among the girls in the class. Sadly, You are NOT ALLOW TO USE THEM AS A NAIL POLISH, 

Now, lets start~~~~

After you had flame dry your slides~ placed them on a rack so that they are easily accessible. 

Apologize since i forget to took photos for crystal violet addition. The photo above shows the addition of iodine into the slides,the volume should just enough to cover your culture, the person is a bit wasteful~~~(Not me not me！)

The iodine were left onto the slides for 30 seconds and was rinsed always with water.

next, the acetone. Acetone is used as a decolorizer so that person washed off the thing directly before i was able to take any photos ~

Now, safranin is added onto the slides and wait.........................................................
..........................

................

.........

..........

..........

........

......poof~30 seconds over

wash wash wash~~~

so~they slides were dried using a paper towel, and a cover slides was added~~

now you complete your gram stain~they should look roughly like those listed above..

Water droplets on the rack~~~pretty~??wahahha

OK~!!!!! that all for today

enjoy your bluish Monday everyone

 

Microbes Theoretical result ~~~part 2

Good morning everyone~

7days to go for our practical exam, since not everyone turn up for today's microbiology lab session, i think is always better if i could post some photos about the theoretical result here, which most of them will be included in our practical exam.

 1st of all, sugar fermentation tube.The red color tube on the left indicates that its a alkaline reaction, while the yellow color  on the left indicates that its a acid reaction. Alkaline reaction is usually cause by the deamination of protein, while the acid is cause by sugar fermentation.

A MR-VP is a test to identified the fate of glucose by an organism. The red color in a MR test is caused by phenol red, which turns red in an acidic condition. This is caused by the acidic fermentation of glucose by the bacterium. Vp test in the other hand, is to identified the production of acetylmethylcarbinol from glucose. Usually 2 reagent needed to be added to the tube before a VP result can be observed. 

IN a OF medium, two separate tube is inoculated with the same bacteria. One is left open while the other is sealed by para-film to create a anaerobic environment. The Positive and negative result is shown as the picture above.

Amylase test is a test for the presence of amylase within the bacteria. Amylase is a enzyme that hydrolyze starch into glucose. A iodine reagent is added to the agar, a clear zone around the colony indicates a positive reaction. 

OK. motility test is a test to determine if the bacteria if moving within the agar. A sharp edge of the colonies indicates a negative result.

Gelatinase is an enzyme that liquefy gelatin (a agar like substances), the liquification of agar shows a positive result.

Catalase test will give out bubbles when hydrogen peroxide is added to the colonies. It is usually present in aerobic bacteria.

The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. A pink ring on top of the agar indicates a positive result.

An oxidase is any enzyme that catalyzes an oxidation-reduction reaction involving molecular oxygen (O2) as the electron acceptor. A blue color indicates a positive result.  

Mannitol salt agar or MSA is a commonly used growth medium in microbiology.

It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective forStaphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria. A mannitol fermenting bacteria such as S.aureus will turn the agar into a yellow color.

• Blood agar plate (BAP) Contains mammalian blood (usually sheep or horse), typically at a concentration of 5–10%. BAP are enriched, differential media used to isolate fastidious organisms and detect hemolytic activity. As seen as above, the hemolytic activity  can be classied as alpha beta and gamma, as shown as above.

NOw, S.aureus on a baird parker medium. Black circular colonies.

OK~this is how you classfied ALpha, beta and gamma(non-hemolytic) activity.

Optochin test, only K.pneumonia will be sensitive towards this antibiotic.As shown as the on the right.

Eosin methylene blue (EMB) is a selective stain for Gram-negative bacteria. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1The morphology of Proteus vulgaris and C.coli on EMB agar. 

A more organism on EMB agar.Enterobacter aerogenosa shows black colonies.

MacConkey agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. A lactose fermenter as shown above, turn the agar into a yellow hue.

More bacteria on MacConkey agar. 

Last one, a microscopic view of Bacillus cereus after spore staining. The green color indicates the presences of endospore.

Thx all for the time, good luck in your exam

see you soon~~

SSBC STA, F&N

 

Good morning everyone~~last Saturday, Swinburne Sarawak Biotechnology Club had Organize a education trip to both Sarawak Timber Association and  F&N soft drink factory

 The picture above shows the situation where some people are boarding the bus and some people are waiting.The journey starts at about 9am, luckily everyone was on time~~~

 

Yes, i am the last to board, no just to take photo, but also to get a view of out pretty MPH(the Green ellipse at the background)

 Everyone is given a bottle of FREE mineral water~!!!! Swinburne brand~~~ 

Now, after half an hours of bus trip, we finally arrived at STA(Sarawak Timber Association) , is a glass covered building with a curve.Basically everything within this building is decorated with greenish stuff...like trees, table cloth and people with greeen hair~!!!

A spiral staircase, you can roll down from the 5th floor if you want to

A glass Rooftop

And another glass rooftop, green house effect can be demonstrate here

A view of STA logo 

I shall skip the boring part of the tack, which is mainly about how much they sell each year for the forest resources  , and the use of this region in biotechnology and how they have help in scholarship and research funding~~

conclusion, hardwood and softwood is determined by the species not the hardness or density

so, hardwood can be very soft somehow

Next, we went to the exhibition hall of STA, which consist of some item unique to the Sarawak forest, mainly all the types of wood, and the usages

 Wood can be made into shoes~~~it is very nice to see~and nice to hold

but once broken, considered sold~~!!

A 13th years old timber that represent the shape of my heart~~A large crack can be seen

reminds me of my scar

 Finally, a group photo in the exhibition hall~~ 

the whole talk ends about 3 hours, including the talk, refreshment and hall visit. 

 At about 12pm, is lunch time!~!!!!!~!~

Now, lets march towards our lunch~~

ok~NAsi lemak with rendang, an egg and a couple of salted fish~

 i enjoy even with my ulcer hurting me every single bites

Now, i named this picture "离开地球表面" ~which means let go in english

The sun is bright and our members were really caring among each others

 Next, a picture taken in F&N cenferences hall, sorry for those who are waiting for some image,

i was told that it was illegal to take any inside...boom

Now, back at Swinburne  University at about 5pm in the afternoon,

 Finally, a logo for SSBC=Swinburne Sarawak Biotechnology Club

Have a nice day~~~