Laminar flow work station~

Hello~is me again

todays article gonna be about the famous laminar flow station

A vertical laminar flow workstations are ideal for applications that are not hazardous to the operator but require a clean work area. The compact, light weight design allows for multiple “clean zones” within a laboratory.

The vertical laminar flow workstation provides clean Class 100 laminar flow air for process protection by using a two stage filtration process. Room air is first cleaned with an electrostatically charged prefilter and then further cleaned with a HEPA filter. AirClean Systems vertical laminar flow workstations have been independently certified to meet or exceed applicable clean bench standards.(credits to air-clean system)

1st of all~an overview of a Ultra pure grade SDS 10%

with the laminar flow as our background...

A photo of SDS and laminar flow~i heard that once SDS killed  laminar flow's father..shhh

Gelman class 100 series laminar flow~just in case, gelman is the brand, not a person covered in gel 

The is also a laminar flow from a view of a 5 years old child~

Obviously, this laminar flow need power to run~and i have no idea why it needs this

Control panel for fan control, UV, and misc

shinny interior~~

UV tube within the laminar flow

hmmm, what does this do?

please tell me what is this someone~and stand a chance to win a trip to swinburne~~~!!

wahaha...jk

see you next time bye~~~

Micro pipettes ~

 Hello~~everyone

today tour is about micro pipette~

Pipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micro pipette can range from less than 1µl to 1000µl (1ml), while macro pipettes can measure volumes greater than 1ml.

 and

Today is all about Micro pipettes~~

 

A array of Micro pipettes ranging from 10 to 1000uL



From right: 10uL, 100uL and 1000uL pipettes, actually they was suppose to be a stand that holds all this pipettes, but this photo was taken at night, and i don't wish to wake them up~

so i just shoot while they were lying down

1000uL micro pipettes

It has a working volume of 100~1000uL, as stated on the top of the pipettes

shhhhhhh.......don't wake them~!!!! they spill acid at you when they wake up

 

100uL Micro pipettes

As above this pipettes is capable of taking a volume from 10~100uL

1000uL pipette tips

So this is the tips of the 1000uL pipettes~it is usually disposable, and the different in height here is caused by the mixing of different brands~~

and this is another box of pipette tips~also used for 1000uL micro pipettes

(and yes~all large one uses blue color as they represent the large ocean,, i do not made this story up, Brandon told me about it)

Pipette tips for 100uL micro pipettes

AND the yellow one here is used for 100uL pipettes~with a yellow representing the land

so now ~~since the working volume of a blue pipettes is from 100-1000uL

how does it actually works?

easy~just turn the nob above to the a value you desire~

but make it as gentle as possible~they are high fragile and if you really hurt them

they would pee themselves and we all will be in trouble

so now~this one is in 1001uL~~

(which turning a value above 1000  is not recommended, this is just a demonstration. i am not gonna use this pipette at this volume)

Brandon forced me to do this also~!!!

so by turning the nob~~i changes the value to 963uL~~

amazing right?

ok now really..

and next is the yellow pipettes～～which i had change the volume from 100uL to 80uL

OK~that's all for today

and that brandon is the stupid ninja in my last post~

hope you don't kill him

he hasn't been trained

Safety features in the lAb

Scientific lab, is a highly Hazardous.

Thats why, lots of safety features are equipped to protect us from any accident.

1st of all, a large and yellowish bio-hazard sign~just to tell people that you had enter a place of no return. 

and of cause, to scare off politician

A friendly reminder to order people that labcoat, covered shoes and safety glasses is needed in the lab 

AT ALL TIME 

some tips~~~

ok~no food and drink

and i dont think anyone would wanna eat or drink in that kind of environment...

some tissue to wipe up spilled chemical, and a instant hand sanitizer which is not really useful

we always prefer water and soap

a fire blanket for any fire that intent to stay over night at the lab

just to keep them comfy

A fire extinguisher that would chase off any fire that are not willing to pay the hotel fees

A whole body shower. This allow people to wash themselves instantly right after breakfast

we are after all, a 5 star lab

ok~just kidding, this is just for rinsing when acid is spilled on your friend.

seriously, i am no idea what is this

\

A fire tap just in case someone is playing with fire~~

and lots of pipeline for putting off fire~~(at least some of them..)

they are actually nitrogen pipes, compress air pipes and liquid butane pipes here

thats all for today folks

have a nice day

Bio-safety Cabinet-Level 1

Happy Birthday everyone~~in the last day of year 2010

i will be introducing a big and expansive cupboard not used for storing clothings

So would you believe me if i say it is usually not advisable to put anything inside?

ok~for the 1st time, is it true..

A biosafety cabinet (BSC), biological safety cabinet, or microbiological safety cabinet is an enclosed, ventilated workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens in the laboratory. Several different types exist, differentiated by the specifics of construction.

the one we using here just a level one cabinet as we are not handling any pathogenic viruses

1stly, an rough overview of the cabinet~~~not much impressive..

 OK~now for the controls of a bio-safety cabinet~buttons for Ultraviolet light, normal lamp, and air flow control can all be found be here

 Some instruction can be found on the bottom right corner of the cabinet!~ 

 A UV proof glass that able to protect us from dangerous UV light during sterilization

 OK ~the basic of a bio-safety cabinet is to provide bacteria and virus free air flow to the environment,
the picture below shows the filter that helps to clean the air

 Another view of the cabinet from an eye of a kid

 A socket can also be found inside the cabinet to provide electricity to any equipment that have to be used inside the cabinet

an arm rest for those tiring arms during culturing

 Last but not least, a drain valve to remove any liquid that were dropped into the cabinet holes.

Happy new year everyone~~~beware of the ninja that wanders around the school of swinburne

is he a biotech??/

i have no idea

spectrophotometer~~

In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer(a device for measuring light intensity) that can measure intensity as a function of the light source wavelength. (credits to wikipedia)

today, i am going tell you everything regarding this spectrophotometer, which can measure the absorbance and transmittance of light at around 300nm to 700nm. 

the one we using below is from thermo scientific, its a single beam spectrometer using the light path of 1centimeter. 

okok~~this is the front part of the spectrometer, with a bar code to prevent confusion as they all look the same and having the same name. 

this is what happen if you were taller than the table.

and this is what happen when you were taller than the table and you had open the cover.

As always, you will need to switch open the instrument. If your IQ is higher than 140, you will know what to do after looking at the photo below.  

A Fan is equipped on the back of this machine to prevent any overheating. This is specially important when a young female is using the instrument.

Usually， the machine is left on for about 15 minutes for calibration  and initiation.

Meanwhile, lets us move on to the sample preparation. 

A container named "cuvette" is used to load the sample before the analysis, It has a waistline of 1cm, this is especially convenient during beer's law calculation. 

A close up of our shy little cuvette~~dont breath to hard, you might blur her view.

Today i am measuring the concentration of protein in a solution, so i am using this "Biuret reagent"

Another close up of our shy little cuvette, now with the protein～～

Then， the cuvette was placed inside meter as she was too shy for me to take another photo.

A screen like this would usually appear when it was just turn on. Genesys mean how shy the cuvette was and soft Rev means how nice should you treat her.

OK~now for the reading~The absorbance of this machine is 0.085A at 546nm.

Lastly, wish everyone a wonderful Christmas

Even though my Christmas was quite boring(babysitting my niece at home), 

my birthday was wonderful thanks to my awesome friends over there~~

Merry Christmas everyone~~and 

good night