Incubator Oven

HI everyone~

Anyone curious about the "Incucell" in our lab?

Practically, it just like a oven

but a lot more expansive.

It serves the basic purposes of keeping the temperature constant within the oven. This is very important as it creates a nice and healthy environment for the bacteria to grow~

This one was set to 36C, which were suitable for pathogenic bacteria such as E.Coli to growth

Re-calculating~

A Biohazard sign to remind people that bacteria are indeed dangerous and will kill you if not handle properly

so, never life this oven by their handle.
common sense

Now for the interior. a glass windows separated the environment. 

Inside view of the incubator

Some basic specifications

We have one oven reserved for drying glassware, which is tuned at 60 C.  

And another one for media storage. it was tuned at 30C.

OK, everyone, that's all for the incubator.

Will  introduce you guys to the refrigerated oven next time~~


Thanks for watching guys,
Don't forget to share and spread this blog if you really like it~

Pasir Panjang Trip -Part1

Hello～

I went to Pasir Panjang on the 18th of June for a beach cleaning survey～

It was almost awesome except for the humongous amount of plastics bag and bottles found on the beach

so, rather than focus on the ugly part

i think i would just share some of my photos taken during the trip~

Enjoy everyone

OK~ that's all for today everyone

stay tune for Part 2 and 3 coming right up~~

Reverse Transcriptase Polymerase Chain reaction

Hi, everyone

This is part of the aquatic assignment, please enjoy

Thanks for watching and byez~~

Reduce, Reuse and RECYCLE

Hello, Everyone

Since i went to spring (is a supermarket) almost 3 times every week

is very hard not to notice these 2 cute "little" recycle bin

1st of all, i was quite happy to see that there are actually people take action in Recycling

i almost give up hope after seeing the river last time.

There are a total of 2 big bin, which collects paper and Plastics.

Recycling bin in Spring Mall

Brown for Plastic

Blue for Paper

Side view~

Another Side view

Conclusion, is delightful to see how happy those bins were

HAHAHA

Please recycle everyone~~

Save the only Earth we Have

Famine 30 Camp in Swinburne

Hello, i know this post was not really related to Biotechnology,

but since it was organised by Swinburne Sarawak Biotechnology Club

i guess is OK to include it here

hope you guys don'd mind

There will be a DIY Famine30 Camp in Swinburne This Year.

It was only open for students and Staff, so please inform if you are interested or just want informations about the event

Yap, you can find everything you need in this Picture below.

Thanks everyone for your attention~

How to make a Gel? (for electrophoresis)

 Hello everyone

Hope you have done well in the exam, 

Wish as HD for YOU~~~~(Sincerely)

So i suppose everyone knows about DNA Gel electrophoresis, and today

i am gonna show you all how to actually make the gel out of powder and water~

you will need a Agarose Gel powder 1st

Some concentration TE buffer, but we use TAE most of the time
which is TE + acetic acid
It is used to stabilized to DNA and prevent degradation

a 100mL measuring cyclinder

and lastly the gel tank, basically a container.

now you need to weight the gel~
for 1% gel, 1g of agarose powder will do

weighting~that's not my hand

and mix 10ml of Concentration buffer with 90ml of water

then just pour the solution into the powder, and try to swirl the beaker to dissolve the powder

and you sent it into a microwave oven

heat for 30 seconds at high power!!

it should be quite warm after the 1st heating, and it is recommend to 

swirll it for a while since microwave does not mixed the solution well

after a few heating cycles, a clear solution should be obtained
it should be almost boiling by now.

then you cool the solution by running it under a cool tap water, or just put into a 60 C water bath for half an hour.

finally, pour the agar into the tank~make sure you don't create bubble
they does not look nice on agar.
 I am sure you don't want pimple on your face, same theory applies

Don't move or play with the tank after you pour in the solution!!!!!!!!!!!!!!
it will result into a rough surface, which also not really that nice to see
and might affect the result later on during DNA analysis

finally, you load in the DNA into the wells, and you run in this matter~~~~






Bye everyone
Have a nice holiday~~~~`