How to make a Gel? (for electrophoresis)

 Hello everyone

Hope you have done well in the exam, 

Wish as HD for YOU~~~~(Sincerely)

So i suppose everyone knows about DNA Gel electrophoresis, and today

i am gonna show you all how to actually make the gel out of powder and water~

you will need a Agarose Gel powder 1st

Some concentration TE buffer, but we use TAE most of the time
which is TE + acetic acid
It is used to stabilized to DNA and prevent degradation

a 100mL measuring cyclinder

and lastly the gel tank, basically a container.

now you need to weight the gel~
for 1% gel, 1g of agarose powder will do

weighting~that's not my hand

and mix 10ml of Concentration buffer with 90ml of water

then just pour the solution into the powder, and try to swirl the beaker to dissolve the powder

and you sent it into a microwave oven

heat for 30 seconds at high power!!

it should be quite warm after the 1st heating, and it is recommend to 

swirll it for a while since microwave does not mixed the solution well

after a few heating cycles, a clear solution should be obtained
it should be almost boiling by now.

then you cool the solution by running it under a cool tap water, or just put into a 60 C water bath for half an hour.

finally, pour the agar into the tank~make sure you don't create bubble
they does not look nice on agar.
 I am sure you don't want pimple on your face, same theory applies

Don't move or play with the tank after you pour in the solution!!!!!!!!!!!!!!
it will result into a rough surface, which also not really that nice to see
and might affect the result later on during DNA analysis

finally, you load in the DNA into the wells, and you run in this matter~~~~






Bye everyone
Have a nice holiday~~~~`