Reverse Transcriptase Polymerase Chain reaction

Hi, everyone

This is part of the aquatic assignment, please enjoy

Thanks for watching and byez~~

Reduce, Reuse and RECYCLE

Hello, Everyone

Since i went to spring (is a supermarket) almost 3 times every week

is very hard not to notice these 2 cute "little" recycle bin

1st of all, i was quite happy to see that there are actually people take action in Recycling

i almost give up hope after seeing the river last time.

There are a total of 2 big bin, which collects paper and Plastics.

Recycling bin in Spring Mall

Brown for Plastic

Blue for Paper

Side view~

Another Side view

Conclusion, is delightful to see how happy those bins were

HAHAHA

Please recycle everyone~~

Save the only Earth we Have

Famine 30 Camp in Swinburne

Hello, i know this post was not really related to Biotechnology,

but since it was organised by Swinburne Sarawak Biotechnology Club

i guess is OK to include it here

hope you guys don'd mind

There will be a DIY Famine30 Camp in Swinburne This Year.

It was only open for students and Staff, so please inform if you are interested or just want informations about the event

Yap, you can find everything you need in this Picture below.

Thanks everyone for your attention~

How to make a Gel? (for electrophoresis)

 Hello everyone

Hope you have done well in the exam, 

Wish as HD for YOU~~~~(Sincerely)

So i suppose everyone knows about DNA Gel electrophoresis, and today

i am gonna show you all how to actually make the gel out of powder and water~

you will need a Agarose Gel powder 1st

Some concentration TE buffer, but we use TAE most of the time
which is TE + acetic acid
It is used to stabilized to DNA and prevent degradation

a 100mL measuring cyclinder

and lastly the gel tank, basically a container.

now you need to weight the gel~
for 1% gel, 1g of agarose powder will do

weighting~that's not my hand

and mix 10ml of Concentration buffer with 90ml of water

then just pour the solution into the powder, and try to swirl the beaker to dissolve the powder

and you sent it into a microwave oven

heat for 30 seconds at high power!!

it should be quite warm after the 1st heating, and it is recommend to 

swirll it for a while since microwave does not mixed the solution well

after a few heating cycles, a clear solution should be obtained
it should be almost boiling by now.

then you cool the solution by running it under a cool tap water, or just put into a 60 C water bath for half an hour.

finally, pour the agar into the tank~make sure you don't create bubble
they does not look nice on agar.
 I am sure you don't want pimple on your face, same theory applies

Don't move or play with the tank after you pour in the solution!!!!!!!!!!!!!!
it will result into a rough surface, which also not really that nice to see
and might affect the result later on during DNA analysis

finally, you load in the DNA into the wells, and you run in this matter~~~~






Bye everyone
Have a nice holiday~~~~`

MIliQ water-water so pure you cannot drink!!

You need a ultra pure water but don't know how to purify?
MiliQ water is the answer!!!!!!

Milli-Q refers to water that has been purified and deionized to a high degree (typically 18.2 MΩ·cm) by a water purification systems manufactured by Millipore Corporation. It is also a registered trademark of Millipore. The term is also used to refer to water produced by any similar system.

A pair of MilliQ filters

Milli-Q water uses resin filters and deionization to purify the water. The system monitors the ion concentration by measuring the electrical resistivity of the water. Higher resistivity means fewer charge-carrying ions. Most Milli-Q systems dispense the water through a 0.22 μm membrane filter.

water tank for miliQ water

It is capable of producing water pure enough to get reproducibility and accuracies within parts per million when using ion mass spectrometers in analytical geochemistry. Other users of this system include microbiology and atmospheric science

so, how to use the ultra pure MiliQ machine to produce ultra-pure miliQ water?

1st, say the password：MiliQ MiliQ MiliQ MiliQ MiliQ MiliQ MiliQ MiliQ MiliQ

and switch on the power and water input for the machine~


wait a few moments, and press the button and show below

Press this button for water

which the water would come out from this "hose"

OK~~that's all you need to do, but don't forget to turn off the power and water after used everyone


that's all for today


bye~~~

Uninterruptible power supply- in the lab!??

Hello  everyone

i know you guys are having exam, that why i wanna introduce you to this thing below

the

UPS, or so called Uninterruptible power supply

it is basically a battery that would served as a back up when there is a power failure

and therefore the name

power kinetics blackout buster tx600

the model we using here is a TX600 series, which would hold a power of about 600W.

more than enough to power your laptop for about one whole day

panel of UPS

so the front panel only have one button and 3 light, this machine can be turn on by pressing down the button once

and can be turn off by holding it for at least 5 seconds

the light in the middle(green light) means that it is running on AC power

the one on the left(red) indicates an error in current, possibly an overload of current.

the one on the right (orange), means that this thing is running on its battery power

or so called discharging

PS: it will beep very annoyingly every 4 seconds when irunning only on battery power

the back of the ups consist of one socket input( the one on the top). and 4 current output.

It can also serve as a surge protector for your phone and modem

some technical details

and last but not least, the rotary evaporator pump in action!!!!

wahahaha

thats all everyone

have a nice  and un-interruptible day

Advance Biochemistry videos

HI, everyone

hope you are enjoying your exam

i had found a few video which might be helpful for people like me taking advance biochemistry

So, enjoy~

See you next time everyone(after exam )

hehe